寄生植物锁阳种子萌发方法及愈伤组织、初生吸器诱导研究

In vitro Germination,Callus Induction and Primary Haustorium Organogenesis in the Parasitic Plant Cynomorium songaricum

Xin YUE, Gui-Lin CHEN

Fig.1 Development of C.songaricum embryo in different periods of cultivation Seeds cultured for 0, 20, 30 and 40 d were examined.The embryos were obtained by crushing the seeds with another microscope slide, transferred to the formalin-aceto-alcohol(FAA) stationary liquid medium for 24 h, and observed with bright ?eld illumination(Fig.1A,C and D). The embryo was stained with a fluorochrome and observed with a fluorescence objective lens(Fig.1B)